2023 Fiscal Year Final Research Report
Biofilm formation and gastric acid resistance capacity in antimicrobial-resistant E. coli ST131.
| Project/Area Number |
21K08499
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 54030:Infectious disease medicine-related
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| Research Institution | Tenri University (2023)
Tenri Health Care University (2021-2022) |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
小松 方 天理医療大学, 医療学部, 教授 (00626814)
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| Project Period (FY) |
2021-04-01 – 2024-03-31
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| Keywords | Escherichia coli / ST131 / YahO / タンパク質間相互作用解析 / 全ゲノム解析 / RNA-Seq解析 |
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| Outline of Final Research Achievements |
The aim of this study was to clarify the function of YahO, CybC and HdeA among the specific protein amino acid mutations in antimicrobial resistant E. coli ST131 discovered in our previous report. Focusing on biofilm formation and acid resistance capacity, mRNA expression quantification and various phenotypic tests were performed, but no particularly significant results were obtained. On the other hand, in pull-down assays with high expressing strains, only YahO E34A detected HchA proteins, suggesting protein-protein interactions. In addition, whole-genome and RNA-seq analyses revealed several characteristic gene expressions that were highly expressed when ST131 pandemic clade C1-M27 was grown in low-nutrient culture.
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| Free Research Field |
臨床微生物学、抗菌薬耐性菌
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| Academic Significance and Societal Importance of the Research Achievements |
本研究で得られた新知見は、近年世界的に懸念されている抗菌薬耐性菌の蔓延のメカニズムを解明する一助となる。
特に今回得られた新知見のうち、ST131が低栄養培地において他のクローンには持ち備えていない特殊な生存戦略メカニズムを解明するかもしれない。大変重要な研究データとなる。
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