Elucidation of the genome replication mechanism of Toga and Matona viruses by host-virus interactome analysis

2023 Fiscal Year Final Research Report

• Project/Area Number: 21K08500
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 54030:Infectious disease medicine-related
• Research Institution: National Institute of Infectious Diseases
• Principal Investigator: Sakata Masafumi 国立感染症研究所, ウイルス第三部, 主任研究官 (20600547)
• Project Period (FY): 2021-04-01 – 2024-03-31
• Keywords: 風疹ウイルス / ゲノム複製機構 / 近接ビオチン化酵素 / マトナウイルス

Outline of Final Research Achievements

Viruses express their genes in infected cells and efficiently replicate their genomes through interactions between viral proteins and various cellular proteins. In this study, we fused an enzyme that adds biotin to neighboring proteins with p150, a nonstructural protein responsible for rubella virus genome replication. We comprehensively identified cellular proteins in the vicinity of the p150 by purifying the biotinylated proteins and performing mass spectrometry analysis. The interaction network between these proteins and the p150 was analyzed to evaluate the contribution of individual cellular proteins to genome replication.

Free Research Field

ウイルス学

Academic Significance and Societal Importance of the Research Achievements

本研究により、ウイルス感染における細胞内タンパク質の役割について重要な知見が得られた。特に、風疹ウイルスのゲノム複製に関与する細胞内在性タンパク質の同定は、ゲノム複製機構の理解に繋がると考えれる。ゲノム複製における細胞内在性タンパク質の相互作用機序の理解は、さらに将来の治療・予防戦略の開発に貢献する可能性がある。