Amelioration of acute lung injury by lung mesenchymal stem cell and carbon monoxide.

2023 Fiscal Year Final Research Report

• Project/Area Number: 21K08912
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 55040:Respiratory surgery-related
• Research Institution: Jikei University School of Medicine
• Principal Investigator: Ohtsuka Takashi 東京慈恵会医科大学, 医学部, 教授 (40306717)
• Co-Investigator(Kenkyū-buntansha): 下田 将之 慶應義塾大学, 医学部(信濃町), 准教授 (70383734) ; 橋本 浩平 公益財団法人がん研究会, 有明病院 呼吸器外科, 医長 (70464964)
• Project Period (FY): 2021-04-01 – 2024-03-31
• Keywords: acute lung injury / mesenchymal stem cell / lung transplantation

Outline of Final Research Achievements

We successfully extracted and cultured mesenchymal stem cells derived from mouse lungs, establishing a stable model for mesenchymal cell culture. The optimal ischemia and reperfusion times for a mouse lung ischemia-reperfusion model were investigated. We examined ischemia of the left lung for 1 hour followed by reperfusion for 2 hours and ischemia of the left lung for 1 hour followed by reperfusion for 3 hours. In the mouse lung ischemia-reperfusion model, no significant differences were observed in the changes in inflammatory cytokines and related mRNA in the lungs between the groups with 1 hour of ischemia/2 hours of reperfusion and 1 hour of ischemia/3 hours of reperfusion. Therefore, we adopted the 1-hour ischemia/2-hour reperfusion model as the lung ischemia-reperfusion model

Free Research Field

Thoracic Surgery

Academic Significance and Societal Importance of the Research Achievements

肺由来の間葉系幹細胞の培養に成功した。これにより肺移植、急性肺障害、などの研究に応用が可能である。動物モデルにおいて間葉系幹細胞による肺疾患の治療に関する基礎研究を行う礎が確立された。