Involvement of the CD69-Myl9/12 system in septic DIC

2023 Fiscal Year Final Research Report

• Project/Area Number: 21K09012
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 55060:Emergency medicine-related
• Research Institution: Chiba University
• Principal Investigator: Miyaychi seiji 千葉大学, 医学部附属病院, 医員 (50895844)
• Co-Investigator(Kenkyū-buntansha): 中田 孝明 千葉大学, 大学院医学研究院, 教授 (20375794) ; 大網 毅彦 千葉大学, 医学部附属病院, 講師 (70527887)
• Project Period (FY): 2021-04-01 – 2024-03-31
• Keywords: 肺血症 / DIC / Myl9 / Covid-19

Outline of Final Research Achievements

In this study, we investigated the involvement of the Myl9-CD69 system in bacterial sepsis. A cecal ligation and puncture (CLP) model was used as a model of sepsis. The involvement of the Myl9-CD69 system was examined by fluorescent immunostaining of the lungs of the CLP model mice to see the Myl9 net, which is a characteristic feature of Myl9 involvement. However, we were unable to obtain the formation of Myl9 net even after changing the severity of the disease. We examined mice with a level of severity of disease that is rarely encountered in general clinical practice, with a mortality rate of 50% death in 24 hours, but we could not confirm the formation of Myl9net.
In addition, during the same period, we measured Myl9 levels in blood in various clinical conditions, and found that Myl9 levels in blood of septic patients were clearly lower.
So we conclude that Myl9 is not involved in the pathogenesis of bacterial sepsis.

Free Research Field

救急集中治療医学

Academic Significance and Societal Importance of the Research Achievements

本研究に先立ち新型コロナウイルス感染症患者におけるMyl9-CD69 systemの関与について研究が行われ、その後血中Myl9濃度と重症度との相関について報告が行われている。
本研究では細菌性敗血症についてMyl9-CD69 systemの関与は確認できず、細菌性敗血症の病態解明や新規治療の開拓には繋がらなかった。しかし、今後も社会的な問題になるであろうCOVID-19の病態と一般的な細菌性敗血症の病態の差異を検討し、特異的な治療を検討していく上での意義はあったものと考える。