Elucidating the Pathophysiology of Post-Cardiac Arrest Syndrome and Developing Novel Therapies through Omics Analysis

2023 Fiscal Year Final Research Report

• Project/Area Number: 21K09029
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 55060:Emergency medicine-related
• Research Institution: Keio University
• Principal Investigator: Sasaki Jinichi 慶應義塾大学, 医学部(信濃町), 教授 (90235250)
• Co-Investigator(Kenkyū-buntansha): 本間 康一郎 慶應義塾大学, 医学部(信濃町), 准教授 (10383762)
• Project Period (FY): 2021-04-01 – 2024-03-31
• Keywords: 心停止症候群

Outline of Final Research Achievements

Background: There are no effective treatments for post-cardiac arrest syndrome (PCAS).
Methods: PCAS animals were resuscitated with extracorporeal cardiopulmonary resuscitation (ECPR), and various parameters were evaluated with hydrogen (H2) gas administration. Results: The H2 group showed improved survival rates and EEG activity. H2 improved brain tissue oxygenation and suppressed the increase in central venous pressure. It also inhibited the increase of Syndecan-1 and increased levels of IL-10, VEGF, and leptin. Omics analysis identified significant changes in d-glutamine and d-glutamic acid metabolism. Conclusion: H2 treatment improved the survival rate and restored brain electrical activity in PCAS animals resuscitated with ECPR. Omics analysis identified potential new therapeutic targets.

Free Research Field

救急医学

Academic Significance and Societal Importance of the Research Achievements

昨今増加している体外循環式心肺蘇生（ECPR）に対して、水素ガス（H2）の投与が生存率と脳機能の回復を顕著に改善することを示した。さらに、オミクス解析による代謝物分析において、ECPR後2時間で両群間にd-グルタミンおよびd-グルタミン酸代謝における有意な変化を同定した。これにより、患者の予後改善、医療コスト削減、新規治療法の開発に貢献する可能性がある。