2023 Fiscal Year Final Research Report

Clarification of the pathology of organ damage after aortic declamping in hemorrhagic shock

Research Project

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Project/Area Number	21K09059
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Multi-year Fund
Section	一般
Review Section	Basic Section 55060:Emergency medicine-related
Research Institution	Nippon Medical School
Principal Investigator	YASUMATSU HIROSHI 日本医科大学, 医学部, 助教 (80614809)
Co-Investigator(Kenkyū-buntansha)	増野智彦日本医科大学, 医学部, 講師 (00318528) 岡田一宏日本医科大学, 医学部, 助教 (40740593) 松本尚日本医科大学, 医学部, 教授 (60242559)
Project Period (FY)	2021-04-01 – 2024-03-31
Keywords	胸部下行大動脈遮断 / 蘇生的開胸術 / 虚血再灌流障害
Outline of Final Research Achievements	In order to clarify the pathology following release of the descending thoracic aorta clamp in severe trauma, this study created a rat model based on actual clinical practice and sought to clarify the pathology. Rats in hemorrhagic shock have an extremely low survival rate and are not viable as an experimental model, so an aortic clamp model was created. Data on hemodynamics and circulatory failure resulting from this model was accumulated. In addition, FLIVO, a fluorescent reagent that specifically binds to caspase, was used to investigate local ischemia-reperfusion injury due to aortic clamping, and an IVIS imaging system was used to capture apoptotic changes in the brain, heart, and lungs. Although no significant results were obtained, the aortic clamp model was a stable model.
Free Research Field	外傷外科学
Academic Significance and Societal Importance of the Research Achievements	大動脈遮断解除後の病態は非常に複雑な要因がからんでいることが予想され、本研究では有意な結果が得られなかった。本研究を今後も遂行していくため、ある程度安定した大動脈遮断モデルは作成できたためここに学術的意義はあった。重症外傷患者の救命率を上げるためには大動脈遮断解除後の病態解明は必要であり、社会的意義があるものと考える。今回の研究では有意な結果は得られなかったものの、今後も研究は継続していくことに意義があると考える。