2023 Fiscal Year Final Research Report
Novel finding of Masquelet technique in rat femoral critical sized bone model
| Project/Area Number |
21K09285
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 56020:Orthopedics-related
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| Research Institution | Juntendo University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
池田 通 東京医科歯科大学, 大学院医歯学総合研究科, 教授 (00211029)
上高原 理暢 東北大学, 環境科学研究科, 教授 (80362854)
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| Project Period (FY) |
2021-04-01 – 2024-03-31
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| Keywords | Masquelet法 / induced membrane / 人工骨 / 巨大骨欠損 / 多核巨細胞 / 再移植 |
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| Outline of Final Research Achievements |
Surgical treatment of large bone defect has been improved since application of induced membrane method (Masquelet method). In this two-step method, bone cement is inserted into the bone defect as a spacer and fibrous capsulation is induced around the wound. Several weeks after the primary treatment, the bone cement is removed, and implantation of bone autografts is performed. In this study, we developed a novel porous calcium phosphate cement and inserted into bone defects created in rats. The grafted portion was covered with polytetrafluoroethylene membrane. Four weeks after, the porous spacer including capillaries and mesenchymal stem cells in it was removed, comminuted and re-implanted with bone autografts. Restoration of the bone defects of this method was fine and acceleration of the healing is found. These results strongly suggest that this novel method is clinically applicable and improve the healing of large bone defects.
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| Free Research Field |
整形外科
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| Academic Significance and Societal Importance of the Research Achievements |
Masquelet法の二期手術において、骨欠損部位に充填する自家骨が不足する場合には、人工骨を添加するが、人工骨の添加が予後に及ぼす影響や最適な人工骨の種類に関する情報は皆無に近い。
本研究では、独自に開発した超多孔質リン酸カルシウムセメントをスペーサーとしてラットの巨大骨欠損モデルに挿入、4週間の留置期間中に骨修復に不可欠な血管と間葉系幹細胞を多孔質材料中に侵入させる。それを抜去、粉砕、二期手術で自家骨と混合し再移植することで、治癒促進と二期手術で必要な自家骨量の少量化が可能となる。研究結果は良好で、本研究成果は実臨床への応用が十分可能であり、骨折治療の飛躍的進歩につながると期待される。
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