Development of a urinary diagnostic kit for Hunner type interstitial cystitis targeting the bladder microbiome

2023 Fiscal Year Final Research Report

• Project/Area Number: 21K09382
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 56030:Urology-related
• Research Institution: Jikei University School of Medicine
• Principal Investigator: Furuta Akira 東京慈恵会医科大学, 医学部, 准教授 (90349613)
• Co-Investigator(Kenkyū-buntansha): 政木 隆博 東京慈恵会医科大学, 医学部, 教授 (60535657)
• Project Period (FY): 2021-04-01 – 2024-03-31
• Keywords: 間質性膀胱炎 / ハンナ病変 / 膀胱内細菌叢 / グラム陽性嫌気性球菌

Outline of Final Research Achievements

L-Tryptophan and IFNγ inhibited the proliferation of bladder epithelial cells, but no inhibitory effect of protein L on the bladder epithelial cell proliferation was observed. Additionally, IFNγ induced CXCL10, but neither L-tryptophan nor protein L did. On the other hand, the expression of protein L, considered one of the virulence factors of GPAC, was investigated in both purchased strains and clinical isolates, but no expression was detected. The drug susceptibility of Anaerococcus and Peptoniphilus isolated, cultured, and identified from patient urine was examined, and they were found to be highly susceptible to ABPC. Therefore, ABPC was administered to the patients with Hunner type interstitial cystitis for two weeks, but no clear improvement in symptoms was observed.

Free Research Field

泌尿器科

Academic Significance and Societal Importance of the Research Achievements

ハンナ型間質性膀胱炎（HIC）とは膀胱に非特異的な慢性炎症を認める疾患と定義されているが、明らかな病因は未だ特定されていない。そこで、本研究では１）HICの病態を解明するため、臨床より分離したGPAC株を用いてHICが誘発されるかをin vitro、in vivoで検証し、２）HICの早期診断に寄与する新規診断バイオマーカーを開発することが目的であった。しかしながら、代表的GPACであるAnaerococcusとPeptoniphilusあるいはプロテインLがHICの病因である可能性は否定され、HICの尿中診断キットの開発は達成できなかった。