Regulatory mechanisms of PSMA expression and its molecular functions in prostate cancer-associated vascular endothelial cells.

2023 Fiscal Year Final Research Report

• Project/Area Number: 21K09427
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 56030:Urology-related
• Research Institution: Ehime University
• Principal Investigator: Miura Noriyoshi 愛媛大学, 医学部附属病院, 講師 (80554427)
• Co-Investigator(Kenkyū-buntansha): 雑賀 隆史 愛媛大学, 医学系研究科, 教授 (10314676) ; 東山 繁樹 愛媛大学, プロテオサイエンスセンター, 教授 (60202272) ; 菊川 忠彦 愛媛大学, 医学部附属病院, 准教授 (70444734)
• Project Period (FY): 2021-04-01 – 2024-03-31
• Keywords: 前立腺癌 / PSMA / 血管内皮細胞

Outline of Final Research Achievements

We found that the addition of a 10,000 g pellet fraction of the culture supernatant of a prostate cancer cell line, LNCaP cells, to the medium of normal vascular endothelial cells, human umbilical vein endothelial cells (HUVECs), induces PSMA transcription. Furthermore, we found that microvesicles secreted by prostate cancer cells induce PSMA expression in surrounding vascular endothelial cells and enhance their angiogenic potential. In addition, we found that only tumor vessels around renal carcinoma were positive for PSMA, suggesting that PSMA may also play an important role in tumor angiogenesis in solid tumors other than prostate cancer.

Free Research Field

泌尿器癌

Academic Significance and Societal Importance of the Research Achievements

前立腺特異的膜抗原 (prostate specific membrane antigen; PSMA)は前立腺癌マーカーとしては確立されているものの、その分子機能や前立腺癌組織形成における機能は殆ど不明である。本研究では、前立腺癌細胞由来microvesicle(MV)による正常血管内皮細胞でのPSMA発現誘導機構とPSMA陽性血管内皮細胞の質的変化を解明することで前立腺癌増殖の新しい知見を得ることができた。