The exploration of late-onset disease infection route of Group B Streptococc us by PCR

2023 Fiscal Year Final Research Report

• Project/Area Number: 21K09525
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 56040:Obstetrics and gynecology-related
• Research Institution: Juntendo University
• Principal Investigator: Makino Shintaro 順天堂大学, 医学部, 教授 (70570894)
• Co-Investigator(Kenkyū-buntansha): 竹田 純 順天堂大学, 医学部, 准教授 (60813459)
• Project Period (FY): 2021-04-01 – 2024-03-31
• Keywords: GBS感染症 / 周産期死亡 / 新生児B群溶血性レンサ球菌感染症 / 等温核酸増幅法

Outline of Final Research Achievements

We aimed to reveal the clinical features to explore transmission route of LOD. Vaginal-and-anorectal specimens were collected from 530 pregnant women at the time of antepartum, delivery or postpartum. Oral/anorectal specimens were collected from their newborns at the time of birth, day3, or day30. Each sample was tested by PCR and/or culture for GBS detection. The DNA for PCR was purified directly from the buffer that the swab specimens were suspended without enrichment cultivation. The antepartum prevalence of maternal GBS was 18.6％ according to PCR, and 16.1％ according to cultures. In 9.6％ of cases, GBS results on PCR were inconsistent with antepartum and delivery. GBS was detected in 4.6％ of neonate even from GBS negative mother. GBS positive neonate with negative mother suggests horizontal infection after birth. Our method is a direct detection without an enrichment cultivation, suggesting the possibility of point of care testing.

Free Research Field

周産期

Academic Significance and Societal Importance of the Research Achievements

現行のガイドラインで予防し得る早発型GBSとは異なり、遅発型GBSは水平感染による出生後感染の可能性が示唆された。したがって本研究で確立した新規核酸検出法は、濃縮培養工程を伴わず直接検出可能であり、分娩時のポイントオブケア検査としてのみならず、発症を疑う新生児の早期診断ツールとしても有効といえ、周産期医療への貢献が期待できる。