Re-vascularization in diabetic retinal ischemia

2023 Fiscal Year Final Research Report

• Project/Area Number: 21K09676
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 56060:Ophthalmology-related
• Research Institution: Kagawa University
• Principal Investigator: Suzuma Kiyoshi 香川大学, 医学部, 教授 (80335265)
• Co-Investigator(Kenkyū-buntansha): 新田 恵里 香川大学, 医学部, 助教 (50771523) ; 逢坂 理恵 香川大学, 医学部, 助教 (60867315) ; 中野 裕貴 香川大学, 医学部附属病院, 講師 (70814854) ; 山下 彩奈 香川大学, 医学部附属病院, 講師 (20448369)
• Project Period (FY): 2021-04-01 – 2024-03-31
• Keywords: VEGF / Angiopoietin / 酸化ストレス

Outline of Final Research Achievements

【Objective】Angiopoietin-1 (Ang-1) is expressed by cells in the perivascular wall and some mesenchymal cells, and regulates vascular structural stability and angiogenesis via Tie2 receptors. Reactive oxygen species (ROS) play an important role in cell apoptosis induced by mechanical stretch stimuli. In this study, we measured and evaluated the expression of Ang-1 in a human Muller cell line in response to a mechanical stretch stimulus, and discussed its relationship to ROS.【Results】The expression of Ang-1 decreased with prolonged mechanical stretch stimulation at 10%/60 cpm, the physiological stretch frequency, for 1, 3, and 6 h, respectively. This decrease in Ang-1 expression was reversed by DPI, an NADPH oxidase inhibitor, and NAC, an ROS scavenger【Conclusion】The stretch stimulus-dependent decrease in Ang-1 expression in Muller cells may be due to increased intracellular oxidative stress caused by activation of intracellular NADPH oxidase.

Free Research Field

眼科学

Academic Significance and Societal Importance of the Research Achievements

現在、Angiopietin2に対する抗体治療が実用化されているが、その治療が有効である病態は詳細にはわかっていない。今回細胞内酸化ストレスと細胞伸展刺激がAngiopoitin1の減少をもたらしたことから、黄斑牽引症候群などが合併する症例で抗体治療がより有効である可能性が示された。