The effect of hydrogen-containing intraocular irrigation fluid on retinal oxidative stress injury associated with vitreous surgery

2023 Fiscal Year Final Research Report

• Project/Area Number: 21K09686
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 56060:Ophthalmology-related
• Research Institution: Nippon Medical School
• Principal Investigator: Takahashi Hiroshi 日本医科大学, 大学院医学研究科, 研究生 (00188046)
• Co-Investigator(Kenkyū-buntansha): 五十嵐 勉 日本医科大学, 医学部, 准教授 (10421190) ; 有馬 武志 日本医科大学, 医学部, 講師 (00823113)
• Project Period (FY): 2021-04-01 – 2024-03-31
• Keywords: 網膜光障害 / 硝子体手術 / 水素

Outline of Final Research Achievements

Exposure to ultraviolet light of a certain intensity and short-wavelength visible light induces retinal damage. Retinal light injury has been frequently reported to occur even with light exposure from ophthalmic instruments.

In this study, we investigated retinal light injury during vitreous surgery in rabbits. Specifically, we performed simple vitrectomy on rabbits and exposed them to xenon light for 30 minutes. We compared the effects of normal irrigation fluid and hydrogen-containing intraocular irrigation fluid. The maximum irradiance was used, with the irradiation site positioned 5 millimeters from the retina and 3 millimeters from the optic nerve head. On the 6th postoperative day, we conducted an electrophysiological analysis using electroretinography (ERG) and analyzed the thickness of the outer granular layer of the retina using optical coherence tomography (OCT). However, no significant differences were observed.

Free Research Field

眼科

Academic Significance and Societal Importance of the Research Achievements

今回の条件では、水素における網膜光障害を減弱することができなかった。今後さらなる検討を要する。