Relationship between chronic wounds and NETs expression for novel therapeutic development targeting NETs

2023 Fiscal Year Final Research Report

• Project/Area Number: 21K09806
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 56070:Plastic and reconstructive surgery-related
• Research Institution: Tokai University
• Principal Investigator: Imagawa Kotaro 東海大学, 医学部, 講師 (50366001)
• Co-Investigator(Kenkyū-buntansha): 増田 治史 医療法人徳洲会湘南鎌倉総合病院(臨床研究センター), 湘南先端医学研究所 再生医療開発研究部, 主席研究員 (50278496)
• Project Period (FY): 2021-04-01 – 2024-03-31
• Keywords: NETｓ / バトロキソビン / 慢性創傷 / 再灌流障害 / 微小循環障害

Outline of Final Research Achievements

In chronic wounds, the expression of Neutrophil Extracellular Traps (NETs) has been confirmed. The expression of NETs may be involved in microvascular damage and delayed wound healing. To investigate whether Batroxobin administration suppresses NETs expression, we utilized a rat skin flap ischemia-reperfusion injury model. We confirmed that Batroxobin administration improves the survival area of the skin flap tissue. Histological examination revealed that the expression of NETs was lower in the Batroxobin-administered group compared to the control group, and a higher number of vessels were observed in the subcutaneous tissue of the Batroxobin-administered group. Batroxobin may possess a microvascular protective effect by suppressing NETs expression.

Free Research Field

形成外科学

Academic Significance and Societal Importance of the Research Achievements

慢性皮膚創傷において局所の強い炎症後に皮膚壊死が生じる原因としてNETｓの発現が関与していることが示唆された。このNETｓによる微小循環障害をバトロキソビンを投与することにより抑制することができることを確認した。バトロキソビンが炎症時の微小循環障害に起因する皮膚組織障害に対し、新たな機序としての治療薬として効果が期待できることを示唆した。