3D High-Speed Rotational Culture for Reconstitution of Human Cartilage Tissue Derived from Human Cartilage Progenitor Cells

2023 Fiscal Year Final Research Report

• Project/Area Number: 21K09812
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 56070:Plastic and reconstructive surgery-related
• Research Institution: Yokohama City University (2023); Kanagawa Children's Medical Center (Clinical Research Institute) (2021-2022)
• Principal Investigator: YASUMURA Kazunori 横浜市立大学, 医学研究科, 共同研究員 (40351621)
• Co-Investigator(Kenkyū-buntansha): 鍵本 慎太郎 横浜市立大学, 附属市民総合医療センター, 助教 (10737480) ; 矢吹 雄一郎 横浜市立大学, 医学研究科, 客員講師 (30610357) ; 小林 眞司 地方独立行政法人神奈川県立病院機構神奈川県立こども医療センター(臨床研究所), 臨床研究所, 部長 (90464536) ; 林 礼人 横浜市立大学, 医学研究科, 教授 (10365645)
• Project Period (FY): 2021-04-01 – 2024-03-31
• Keywords: 軟骨再生 / 軟骨細胞 / 回転培養

Outline of Final Research Achievements

Autologous cartilage tissue transplantation for tissue deformities in the craniofacial region is fraught with problems such as the highly invasive nature of cartilage harvesting and quantitative limitations. Although there are growing expectations for regenerative therapy using human chondrocytes as a new treatment method, it has not been possible to reconstitute cartilage tissue that can be transplanted intact in vitro. Although it has been possible to reconstitute cartilage tissue without using scaffolds in vitro using a low-speed rotational suspension culture technique, the technique is still somewhat immature histologically. To solve this problem, we attempted to reconstitute mature cartilage tissue in vitro using a high-speed rotational culture technique. As a result, a variety of mature cartilage tissues were reconstituted, ranging from films to masses.

Free Research Field

小児形成外科

Academic Significance and Societal Importance of the Research Achievements

世界に先駆けてヒト軟骨細胞を用いた高速回転培養における条件や手技などの基盤技術を確立し日本発の手技として世界に発信することが期待される。