Fundamental research to establish a risk diagnostic method for highly multidrug-resistant Staphylococcus aureus of oral origin.

2023 Fiscal Year Final Research Report

• Project/Area Number: 21K09858
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 57020:Oral pathobiological science-related
• Research Institution: Hiroshima University
• Principal Investigator: MATSUO MIKI 広島大学, 医系科学研究科(歯), 准教授 (20527048)
• Co-Investigator(Kenkyū-buntansha): 小松澤 均 広島大学, 医系科学研究科(歯), 教授 (90253088) ; 菅井 基行 国立感染症研究所, 薬剤耐性研究センター, センター長 (10201568) ; LE NGUYEN・TRA・MI 広島大学, 医系科学研究科(歯), 助教 (20897904)
• Project Period (FY): 2021-04-01 – 2024-03-31
• Keywords: 薬剤耐性 / 黄色ブドウ球菌 / バクテリオシン

Outline of Final Research Achievements

The aim of this study was to verify that endogenous genetic variation affects the drug susceptibility of Staphylococcus aureus, and genomic analysis of approximately 100 isolates of S. aureus showed a common polymorphism in the graRS, dlt and mprF genes, which affected the susceptibility of positive charges to antimicrobial substances, and that this polymorphism was not necessarily consistent with ST classification (Under review). On the other hand, we found four bacteriocins, bacterial-derived antimicrobial factors that were found to be effective against drug-resistant S. aureus. Two of these bacteriocins were from the genus Staphylococcus, which was isolated from humans in the process of isolating S. aureus in the above project, suggesting that some indigenous bacteria are responsible for the elimination of drug-resistant S. aureus.

Free Research Field

細菌学・口腔細菌学

Academic Significance and Societal Importance of the Research Achievements

近年薬剤耐性菌問題が深刻化しており、特に多剤耐性黄色ブドウ球菌 (MRSA) は常在細菌であるとともに、多種多様な病原性因子を持つ病原細菌である。一般的に黄色ブドウ球菌はプラスミド等から外来性に薬剤耐性を獲得することは知られているが、本研究から薬剤耐性はSTに依存せず、特定の遺伝子多型性により影響を受けることが示唆され、この知見は、現在のST分類による薬剤耐性のリスク判定に加え、特定の遺伝子パターンにも着目した解析が必要であることを提案できる。