Differentiation and induction of periodontal tissue constituent cells by transcription factor regulation using miRNA

2023 Fiscal Year Final Research Report

• Project/Area Number: 21K09922
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 57030:Conservative dentistry-related
• Research Institution: Nihon University
• Principal Investigator: TAKAI Hideki 日本大学, 松戸歯学部, 准教授 (30453898)
• Co-Investigator(Kenkyū-buntansha): 小方 頼昌 日本大学, 松戸歯学部, 教授 (90204065)
• Project Period (FY): 2021-04-01 – 2024-03-31
• Keywords: 分化誘導 / miRNA / 転写因子

Outline of Final Research Achievements

To differentiate stem cells into cells with different properties, it is necessary to induce the expression of specific transcription factors (TFs). MicroRNAs (miRNAs) bind to the 3'-UTR of mRNA and cause translational repression. We analyzed the type of cells that are induced to differentiate into gingival fibroblast (HGF) by suppressing TFs that are highly expressed in periodontal tissue using miRNA. miR200a bound to Twist2 3'-UTR and increased DLX5 and RUNX2 mRNA and protein levels. These results suggested that miR200a may induce differentiation of HGF into osteoblasts.

Free Research Field

歯周病学

Academic Significance and Societal Importance of the Research Achievements

本実験では歯周病治療の実用化を目指して、ヒト歯周組織構成細胞に特異的な転写因子の発現をmiRNAで調節するところが独創的であると思われる。予想される結果としてヒト歯周組織構成細胞を異なる性質の細胞に分化誘導されると考えられる。以上のことから，本研究の意義は，歯周組織構成細胞に発現する特異的な転写因子をmiRNAで調節することにより、将来の歯周治療に応用することである。