Bone reproduction instruction technology development of bone defect due to the undifferentiated cell and tissue body around the mold

2023 Fiscal Year Final Research Report

• Project/Area Number: 21K09931
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 57040:Regenerative dentistry and dental engineering-related
• Research Institution: The University of Tokyo
• Principal Investigator: Uchida Yoko (大山洋子) 東京大学, 医学部附属病院, 届出研究員 (40897857)
• Co-Investigator(Kenkyū-buntansha): 古村 眞 東京大学, 医学部附属病院, 特任教授 (10422289)
• Project Period (FY): 2021-04-01 – 2024-03-31
• Keywords: 骨分化誘導 / 骨再生

Outline of Final Research Achievements

Objective: We aimed to develop a less invasive therapeutic method for treating cleft lip and palate in the bone defect region. Method:We created a 3.5mm diameter loss model on a mouse skull. We inserted a silicon mold into the periosteum on the affected side.
The mold was surrounded by an encapsulation body. Results: The encapsulation body around the mold showed positive periosteum organization, confirmed by alkaline phosphatase and alizarin red staining. Interestingly, this organization was not observed under the skin. Additionally, we found that bone reproduction around the mold was promoted in mice with cerebral damage.

Free Research Field

再生医療

Academic Significance and Societal Importance of the Research Achievements

本研究の成果は、骨再生が自律的に促進されることを確認することができた。骨欠損部に鋳型を置くことでその周囲に総省治癒早期に線維性組織が被覆し、その組織が骨膜類似組織となることが示唆された。また、骨再生の再現性が低く、論文化、知財化まで3年間で到達できなかったが、さらなる因子を加えることで骨再生の促進が得られることが確認された。もう一つのファクターを見出すことで、より低侵襲で確実な骨欠損部への骨再生技術が開発されるものと考えられる。