Mechanism of undifferentiated maintenance immature osteoblasts under microgravity environment

2023 Fiscal Year Final Research Report

• Project/Area Number: 21K09944
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 57040:Regenerative dentistry and dental engineering-related
• Research Institution: Kanagawa Dental College
• Principal Investigator: Handa Keisuke 神奈川歯科大学, 歯学部, 教授 (40433429)
• Co-Investigator(Kenkyū-buntansha): 齋藤 正寛 東北大学, 歯学研究科, 教授 (40215562) ; 二瓶 智太郎 神奈川歯科大学, 大学院歯学研究科, 教授 (50237781)
• Project Period (FY): 2021-04-01 – 2024-03-31
• Keywords: 細胞分化 / 微小重力環境

Outline of Final Research Achievements

In this study, we aimed to control osteoblast differentiation under microgravity by examining gene expression and calcium deposition in mouse osteoblast-like cells, KUSA, using Alizarin Red staining, and to develop a bone filler that combines osteoblasts with a 3D collagen scaffold created using a 3D printer in a bone defect state. As a result, we were able to control cell differentiation in a microgravity environment and achieve research that will lead to the development of a 3D collagen scaffold.

Free Research Field

保存修復学

Academic Significance and Societal Importance of the Research Achievements

本研究成果によって、重力と骨芽細胞の分化機構の詳細が明らかになることによってこれまで人工的に薬剤で分化制御を行なってきたが、重力による細胞自身の分化制御機構を利用することによって骨粗鬆症や骨折後の治癒促進剤の新規創薬に繋がる。