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2023 Fiscal Year Final Research Report

Spatiotemporal analysis of regulatory mechanisms for bone modeling and remodeling by Wnt signaling

Research Project

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Project/Area Number 21K10085
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 57060:Surgical dentistry-related
Research InstitutionThe University of Tokyo

Principal Investigator

Hikita Atsuhiko  東京大学, 医学部附属病院, 特任教授 (60443397)

Co-Investigator(Kenkyū-buntansha) 阿部 雅修  東京大学, 医学部附属病院, 講師 (10392333)
星 和人  東京大学, 医学部附属病院, 教授 (30344451)
Project Period (FY) 2021-04-01 – 2024-03-31
Keywords骨リモデリング / 骨モデリング / カップリング / Wntシグナリング / イメージング / In vitro系
Outline of Final Research Achievements

A fluorescence probe was constructed for the detection of Wnt signaling activity. In a culture system in which bone modeling and remodeling could be reproduced and observed temporally by two-photon microscopy, the relationship between osteoblast morphology and matrix change was elucidated. In addition, the re-activation process of osteoblasts by stimulation of Wnt signaling was analyzed quantitatively. Furthermore, in bone remodeling process, spatiotemporal relationship between matrix resorption and formation, which is called coupling, was indicated quantitatively, which was disappeared by inhibition of cathepsin activity.

Free Research Field

骨代謝

Academic Significance and Societal Importance of the Research Achievements

本研究では独自の培養系を用いて、骨の恒常性維持において重要とされるWntシグナル活性と骨芽細胞や基質変化の関係を時空間的に明らかにした。また、骨の吸収と形成の関連を解析することは困難であるが、本研究では独自の培養系によりこの現象を捉え、また薬剤投与によりこの現象が影響を受けることを示した。本研究で得られた成果は、骨恒常性維持機構の理解を深め、よりよい骨粗鬆症治療薬の開発につながることが期待される。

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Published: 2025-01-30  

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