Development of graft material for treatment of osteonecrosis of the jaw with angiogenesis

2023 Fiscal Year Final Research Report

• Project/Area Number: 21K10094
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 57060:Surgical dentistry-related
• Research Institution: Hiroshima University
• Principal Investigator: Nakagawa Takayuki 広島大学, 医系科学研究科(歯), 助教 (30456230)
• Co-Investigator(Kenkyū-buntansha): 武知 正晃 独立行政法人国立病院機構(呉医療センター臨床研究部), その他部局等, 医師 (00304535) ; 太田 耕司 広島大学, 医系科学研究科(歯), 教授 (20335681)
• Project Period (FY): 2021-04-01 – 2024-03-31
• Keywords: 薬剤関連顎骨壊死

Outline of Final Research Achievements

The aim of this study was to establish a method for prevention and treatment of osteonecrosis of the jaw through angiogenesis by inducing differentiation of a sufficient amount of vascular endothelial cells from iPS cells on interconnected porous hydroxyapatite (IP-CHA) and transplanting them into the lesion area of osteonecrosis of the jaw. iPS cells were maintained in feeder cells, MEF The iPS cells were maintained in the presence of feeder cells, MEFs, and bFGF, and induced to differentiate into mesoderm with Activin and BMP4, and finally into endothelial cells with VEGF. However, cells differentiated into endothelial cells were not observed on IP-CHA or on the culture dish. Various conditions for feeder cell culture and colony concentration at passaging were investigated, but conditions that allowed appropriate passaging maintenance were not obtained.

Free Research Field

口腔外科学

Academic Significance and Societal Importance of the Research Achievements

本研究では、IP-CHA存在下でのiPS細胞の血管内皮細胞への分化誘導が困難であり、in vitroでの移植材料の開発ができなかった。この結果は、IP-CHAが細胞培養液の構成成分の吸着に加え、フィーダー細胞からもたらされる成長因子や分化誘導因子にも影響を与えることにより、通常の培養ディッシュ上での分化誘導とは異なる条件検討が必要である。このことは、in vitroで培養したiPS細胞由来の移植材料が、移植後の生体内で期待される結果をもたらすかは、in vivoでの移植実験が重要であることを示唆している。