Challenges to create human dentin organoids with functional odontoblast.

2023 Fiscal Year Final Research Report

• Project/Area Number: 21K10176
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 57070:Developmental dentistry-related
• Research Institution: Fukuoka Dental College
• Principal Investigator: OKA KYOKO 福岡歯科大学, 口腔歯学部, 教授 (60452778)
• Project Period (FY): 2021-04-01 – 2024-03-31
• Keywords: スフェロイド / バイオ3Dプリンター / Tenascin C / DMP1 / 象牙質 / 象牙芽細胞

Outline of Final Research Achievements

The aim of this study was to create a 3D construct as a model of the dentin-pulp complex. 3D construct created using O9-1 cells’spheroid, cranial neural crest cell line from mice. The 3D construct was created by skewering spheroids using KENZAN bio-3D printer. Cell proliferation area and tenascin C and DMP1 expressions were observed in the outer layer of spheroids. In addition, expressions of tenascin C and DMP1 in spheroids were significantly enhanced compared to two-dimensional cultures. In the 3D construct created by the bio-3D printer, cell proliferation regions and tenascin C expression were confirmed on the outside of the construct, although little DMP1 expression was observed. Interestingly, in a 3D construct cultured in calcification induction medium, DMP1 expression was promoted, and DMP1-positive cells existed in the outermost layer, without overlapping with tenascin C expression.

Free Research Field

小児歯科

Academic Significance and Societal Importance of the Research Achievements

象牙質-歯髄複合体をex vivoで再現することができれば、生体内の歯において、有髄の歯にどのような象牙質修復メカニズムをが存在しているのか、また象牙質修復を活性化する因子の探索を生体に近い状態で解析することが可能になる。
遺伝性疾患である象牙質形成不全症や、齲蝕が外傷などで破損した象牙質に対し、第3象牙質の形成を促すことが出来るようになれば、歯科医療において大きく貢献する。また、象牙質修復を促すための薬理学的作用の検証なども行うことができ、覆髄剤等の開発にも寄与できる。