Investigation of a new therapy for auto-transplantation of developing teeth with NF-kB decoy ODN

2023 Fiscal Year Final Research Report

• Project/Area Number: 21K10179
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 57070:Developmental dentistry-related
• Research Institution: Tokyo Medical and Dental University
• Principal Investigator: ISHIDA YUJI 東京医科歯科大学, 大学院医歯学総合研究科, 助教 (00516297)
• Project Period (FY): 2021-04-01 – 2024-03-31
• Keywords: 矯正歯科学 / 再植 / 核酸医薬

Outline of Final Research Achievements

The aim of this study was to develop a novel treatment method for pulp vital response and maintenance of root formation by adapting NFkB decoys, which are known to promote wound healing in periodontal tissue, to address the issues of tooth dropout, pulp necrosis and impaired root formation due to postoperative inflammation that are challenges when replanting young permanent teeth, and to analyse this in animal experimental model was used to analyse the results. The results showed that administration of NFkB decoys with PLGA as a drug carrier effectively suppressed the initial inflammation of the periodontal ligament and pulp after reimplantation, inhibited osteoclast accumulation, suppressed root resorption, maintained root formation capacity and also promoted tissue regeneration from an early stage. On the other hand, no inhibitory effect of NFkB decoy administration on ankylosis formation was observed.

Free Research Field

矯正歯科学

Academic Significance and Societal Importance of the Research Achievements

永久歯を喪失する主要な原因として、幼少期に限れば、外傷が歯の喪失の主な要因となる。外傷による脱臼の保存的治療としては、脱臼歯を抜歯窩に速やかに戻し、固定する再植治療が行われるが、感染、歯槽骨吸収、歯根吸収、アンキローシスなど様々な術後の問題点が報告されている。しかし、いまだそれらを防ぐ有効な治療法が確立していない。
本研究結果は、幼若永久歯の脱臼時に再植治療を適応した際に、NFｋBデコイなどの抗炎症薬を応用することで、再植後の初期炎症を抑えることが、早期からの創傷治癒を促進し、歯根吸収および歯根形成能の維持に寄与することが示唆された。今後本研究を発展させ、新しい治療法を開発していきたい。