Drug Screening and Metabolome Analysis Using Orbitrap LC-MS/MS

2023 Fiscal Year Final Research Report

• Project/Area Number: 21K10514
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 58040:Forensics medicine-related
• Research Institution: Asahikawa Medical College
• Principal Investigator: Okuda Katsuhiro 旭川医科大学, 医学部, 助教 (00389115)
• Project Period (FY): 2021-04-01 – 2024-03-31
• Keywords: Orbitrap LC-MS/MS / メタボローム / 薬毒物スクリーニング

Outline of Final Research Achievements

In the development of a drug screening method employing Orbitrap LC-MS/MS, analytical conditions were optimized using a mixed-mode column, permitting dual elution times without necessitating a column change. Under this condition, drug screenings were conducted for forensic autopsy evaluations, amassing scan data for approximately 800 cases. A thorough analysis of these data by cause of death unveiled several compounds exhibiting decreasing or increasing trends contingent upon the cause of death. However, significant challenges remained in terms of specificity and eliminating the effects of decomposition.

Free Research Field

法中毒学

Academic Significance and Societal Importance of the Research Achievements

本課題で確立した分析条件は、高極性化合物から低極性化合物まで幅広く保持・分離することができ、スキャンデータであることから事後に様々な解析が可能である。今後もデータを蓄積していくことで、死因診断マーカー発見の重要な礎になると考えられる。