2023 Fiscal Year Final Research Report
Development of high-sensitive detection method for compounds taken even decades ago, using human synovial fluid or cartilage specimens
| Project/Area Number |
21K10519
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 58040:Forensics medicine-related
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| Research Institution | Hamamatsu University School of Medicine |
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Principal Investigator |
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| Project Period (FY) |
2021-04-01 – 2024-03-31
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| Keywords | 関節液 / 関節軟骨 / LC-MS/MS / 超高感度分析 |
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| Outline of Final Research Achievements |
This study aim to develop a novel and reliable analytical method for detecting and measuring target xenobiotic(s) administrated far before (up to years) and then contained in various tissues of human joints, such as intervertebral discs and synovial fluids, in forensic cases; it would also be expected to carry out the analysis without contaminations owing to robust and compact anatomical structure of the human joints. Distribution of triazolam and its major metabolite hydroxy-triazolam in synovial fluid, femoral vein and heart blood, cerebrospinal fluid, pericardial fluid, bile and stomach contents obtained from an authentic intoxication case could be determined. In addition, comprehensive review on quantitative methods employed in forensic toxicological field, including matrix matched calibration method, standard addition method and surrogate analyte approach method with their validation, was carried out and published as a review article.
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| Free Research Field |
法中毒学
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| Academic Significance and Societal Importance of the Research Achievements |
法医学分野では，関節液や関節軟骨を対象とした薬毒物分析について，これまでごく少数の膝関節液での報告のみであった．関節軟骨はそのターンオーバー（入れ替わり）が極めて長く、数十年ともいわれている．これら関節構造での薬毒物の分布・死後再分布についての知見や体系的なデータの蓄積は極めて乏しい．本研究では，膝関節を構成する要素（関節液、軟骨など）を分析対象とした高感度分析方法の開発を試みる．この研究により，コンタミネーションが少なく，かつ検出可能期間がきわめて長期間(数年から数十年を期待)である法医学分野での薬毒物の分析・鑑定方法の開発が期待される．
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