Impact of Veno-arterial Extracorporeal Membrane Oxygenation on Skeletal Muscle Function and PO2 in Contracting Muscle of Normal Rats

2023 Fiscal Year Final Research Report

• Project/Area Number: 21K11177
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 59010:Rehabilitation science-related
• Research Institution: Kitasato University (2022-2023); Niigata University of Health and Welfare (2021)
• Principal Investigator: Hotta Kazuki 北里大学, 医療衛生学部, 准教授 (30791248)
• Project Period (FY): 2021-04-01 – 2024-03-31
• Keywords: ECMO / 骨格筋 / 酸素動態

Outline of Final Research Achievements

The impact of non-physiologic circulation induced by VA-ECMO on blood flow and function of skeletal muscle is unclear. This study aimed to clarify the effects of high fraction of inspiratory oxygen (FiO2) and VA-ECMO on interstitial partial oxygen pressure (PO2) and function of rat skeletal muscle. SD male rats were randomly assigned to control, 100% of FiO2 (100%O2) and VA-ECMO with high FiO2 (ECMO plus 100%O2) groups, respectively. Compared to the control, arterial oxygen pressure were significantly higher in the 100%O2 and ECMO plus 100%O2 groups. The interstitial PO2 at rest and during muscle contractions were significantly lower in ECMO plus 100%O2 than those of the control and 100%O2 groups. The peak tension and relaxation rate of the muscle in ECMO plus high FiO2 group was lowest between groups. These results suggest that VA-ECMO impairs skeletal muscle function and reduces interstitial PO2 in contracting muscle of normal rats.

Free Research Field

循環生理学

Academic Significance and Societal Importance of the Research Achievements

体外式膜型人工肺は積極的な酸素療法，人工呼吸器管理によっても十分な酸素化と循環が保てない場合の標準的な治療法である．体外式膜型人工肺から離脱したあと，元の日常生活に戻るためには歩行を中心とした運動機能の再建が求められる．本研究は体外式膜型人工肺そのものが骨格筋の機能障害を引き起こす原因であることを示唆している．高酸素血症は直接的な原因とはならず，逆に筋組織内の低酸素が関与している可能性がある．