The role of skeletal muscle-derived phenomena in exercise-independent increases in skeletal muscle mass

2023 Fiscal Year Final Research Report

• Project/Area Number: 21K11276
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 59010:Rehabilitation science-related
• Research Institution: Seijoh University
• Principal Investigator: Ohno Yoshitaka 星城大学, リハビリテーション学部, 准教授 (80440808)
• Project Period (FY): 2021-04-01 – 2024-03-31
• Keywords: 細胞・組織 / 骨格筋 / 筋肥大

Outline of Final Research Achievements

The purpose of this study was to investigate the effects of heat and/or lactate stimulation conditions on the anabolic regulation of skeletal muscle mass using cell culture system. Mouse myoblast-derived C2C12 cells were used and differentiated to form myotubes. During the differentiation phase, heat stimulation increased the myotube diameter and the protein content in C2C12 cells. The effect of heat stimulation on muscle mass may depend on the duration of the stimulation. Mild lactate stimulation promoted the differentiation of C2C12 cells. The combination of heat and lactate stimulation upregulated anabolic intracellular signaling. These observations suggest that the combined heat and lactate stimulation may activate protein synthesis and increase muscle anabolism more than either stimulation alone.

Free Research Field

リハビリテーション科学

Academic Significance and Societal Importance of the Research Achievements

本研究は筋由来の現象である熱産生と乳酸分泌に着目し、温熱刺激と乳酸刺激およびその組合せ刺激による筋の同化応答への影響を検討したものである。組合せ刺激はそれぞれの単独刺激よりも筋同化応答を促進する可能性が考えられた。生体内では温熱刺激と乳酸刺激の組合せ刺激のように、複数の筋由来の現象が相互に作用して筋増量に影響を及ぼすと考えられる。本研究の成果は、運動に代わる新たな筋増量法の開発に有益な情報となると考える。