2023 Fiscal Year Final Research Report

New probiotics alter gut microbiota in rat: Aiming to improve gut dysbiosis after gastrectomy for gastric cancer.

Research Project

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Project/Area Number	21K11708
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Multi-year Fund
Section	一般
Review Section	Basic Section 59040:Nutrition science and health science-related
Research Institution	Kyorin University
Principal Investigator	Takagi Taisuke 杏林大学, 医学部, 医員 (60868991)
Co-Investigator(Kenkyū-buntansha)	阿部展次杏林大学, 医学部, 教授 (40266747) 大崎敬子杏林大学, 医学部, 教授 (90255406)
Project Period (FY)	2021-04-01 – 2024-03-31
Keywords	腸内細菌 / 胃切除 / プロバイオティクス / dysbiosis / 体重減少 / 酪酸 / IgA / メタゲノム解析
Outline of Final Research Achievements	Body weight loss after gastrectomy occurs with high frequency. dysbiosis in the gut microbiota has recently been reported as one possible cause. We explore the ability of novel bacteria to prevent weight loss by improving dysbiosis focusing on the rice fermentation bacteria Leuconostoc spp., Probiotic candidates, Leuconostoc suionicum MS009 and Leuconostoc mesenteroides MS061were compared. MS009 was able to increase in “omoyu” for use as probiotics. The adhesion ability of MS009 to INT-407 cells was higher than that of the other two. In an animal experiment of MS009, the cecal microbiota of rats shows that a higher α-diversity and changes of β-diversity. Moreover, increasing of IgA antibodies and butyric acid in cecal content was observed in the treatment group.MS009 was shown to alter the composition of the gut microbiota and promote increased butyrate production, and IgA production. Together, these results indicate that this strain is suitable for use as a probiotic.
Free Research Field	腸内細菌学
Academic Significance and Societal Importance of the Research Achievements	重湯中で増殖、投与が可能な新規プロバイオティクス細菌の性能評価を行った。新規プロバイオティクス候補Leuconostoc suionicum MS009株は、ラット腸内細菌を変化させ、酪酸産生増加作用、IgA抗体産生増加作用を通じて、宿主に有益な効果をもたらすことが予想された。 MS009株はお粥中で投与可能な全く新しいプロバイオティクスとしてお粥を食す病態にある高齢者や手術後の患者に投与し病状を回復させる可能性が展望された。