2023 Fiscal Year Final Research Report
Evolutionary analysis of the nuclear receptors in invertebrates to infer ecological effects of environmental chemicals.
| Project/Area Number |
21K12260
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 63030:Chemical substance influence on environment-related
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| Research Institution | Suzuka University of Medical Science |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
中西 剛 岐阜薬科大学, 薬学部, 教授 (50303988)
松丸 大輔 岐阜薬科大学, 薬学部, 准教授 (50624152)
目加田 京子 岐阜薬科大学, 薬学部, 会計年度任用職員 (40898235)
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| Project Period (FY) |
2021-04-01 – 2024-03-31
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| Keywords | 核内受容体 / レチノイドX受容体 / 有機スズ類 / システイン |
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| Outline of Final Research Achievements |
We cloned the RXR gene from The Mediterranean mussel (Mytilus galloprovincialis). We identified two types of RXR genes: one in which the 465 residue was a cysteine (mgRXRc) and the other in which it was a serine (mgRXRs). The residue corresponds to the 432 residue of human RXRalpha, which is essential for the binding of organotins to human RXRalpha. We investigated the ligand-responsiveness of the two proteins and found that mgRXRc bound to organotins, whereas mgRXRs did not. In addition, we investigated whether the mgRXR was involved in the regulation of gene expression. The data suggested that the mgRXR was involved in the regulation of gene expression in the same as vertebrate nuclear receptors.
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| Free Research Field |
分子毒性学
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| Academic Significance and Societal Importance of the Research Achievements |
本研究成果は、有機スズ類がムラサキイガイに対して核内受容体を介して何らかの影響を及ぼす可能性を示唆するものである。また、ムラサキイガイレチノイドX受容体には遺伝子多型が存在し、個体によって有機スズ類に対する感受性が異なる可能性があることを見いだした。この遺伝子多型と有機スズ類の曝露との関連性についてはさらなる研究が必要と考えられる。これらの成果は、環境化学物質が無脊椎動物に及ぼすリスクを推定する一助となることが期待される。
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