2023 Fiscal Year Annual Research Report
Elucidating transmission dynamics for soil-transmitted helminths based on environmental DNA analysis
| Project/Area Number |
21K12269
|
|---|
| Research Institution | Niigata University of Pharmacy and Medical and Life Sciences |
|---|
Principal Investigator |
オオタケサトウ マルセロ 新潟薬科大学, 医療技術学部, 教授 (50771896)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
迫 康仁 旭川医科大学, 医学部, 教授 (40312459)
サトウ 恵 新潟大学, 医歯学系, 准教授 (70601813)
|
|---|
| Project Period (FY) |
2021-04-01 – 2024-03-31
|
| Keywords | Ecohealth / One-health / environmental DNA / Helminths / qPCR / STH |
|---|
| Outline of Annual Research Achievements |
The STH to be detected were A lumbricoides, T trichiura and Hookworms (A duodenale, and N americanus). From the aligned sequences of cox1 gene by species of parasite we determine specific variable/conserved areas and design sets of primers/probes to be used in multiplex qPCR. The assay was screened in silico for specificity. Worms' DNA were used to attest the usefulness of the system. Then, soil samples from endemic area were used. The soil eDNA was extracted and tested, completing the goals of this research project with the correlation of positive STH cases with the environmental contamination. Furthermore, we are still analyzing more soil samples to increase the number of tests, and use it to design an accurate risk mapping of STH infection sites from endemic areas. Paper in preparation
|
Research Products
(2 results)
