2023 Fiscal Year Final Research Report
Elucidating transmission dynamics for soil-transmitted helminths based on environmental DNA analysis
| Project/Area Number |
21K12269
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 63040:Environmental impact assessment-related
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| Research Institution | Niigata University of Pharmacy and Medical and Life Sciences (2023)
Dokkyo Medical University (2021-2022) |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
迫 康仁 旭川医科大学, 医学部, 教授 (40312459)
サトウ 恵 新潟大学, 医歯学系, 准教授 (70601813)
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| Project Period (FY) |
2021-04-01 – 2024-03-31
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| Keywords | ecohealth / one-health / environmental DNA / helminths / pPCR / STH |
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| Outline of Final Research Achievements |
In this study, the STH to be detected were A lumbricoides, T trichiura and Hookworms (A duodenale, and N americanus). From the aligned sequences of cox1 gene by species of parasite we determine specific variable/conserved areas and design sets of primers/probes to be used in multiplex qPCR. The assay was screened in silico for specificity. Worms' DNA were used to attest the usefulness of the system. Then, soil samples from endemic area were used. The soil eDNA was extracted and tested, completing the goals of this research project with the correlation of positive STH cases with environmental contamination. Furthermore, we are still analyzing more soil samples to increase the number of tests, and use it to design an accurate risk mapping of STH infection sites from endemic areas.
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| Free Research Field |
one-health
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| Academic Significance and Societal Importance of the Research Achievements |
The results showed one sample from a house surrounding where the residents presented positive result for Ascaris lumbricoides (presenting a relationship on environment and human parasitic status). This is the first study showing the usefulness of eNA in soils samples for determination of STH.
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