Bioassay sensor using stimuli-response of immobilized model cell membranes

2023 Fiscal Year Final Research Report

• Project/Area Number: 21K12283
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 64010:Environmental load and risk assessment-related
• Research Institution: Wakayama National College of Technology
• Principal Investigator: Morita Seiichi 和歌山工業高等専門学校, 生物応用化学科, 准教授 (70332054)
• Co-Investigator(Kenkyū-buntansha): 土井 正光 和歌山工業高等専門学校, 生物応用化学科, 教授 (30217608) ; 西本 真琴 和歌山工業高等専門学校, 生物応用化学科, 准教授 (70609057)
• Project Period (FY): 2021-04-01 – 2024-03-31
• Keywords: モデル細胞膜 / 脂質二分子膜 / バイオアッセイ / 環境評価 / バイオセンサー / 熱分析

Outline of Final Research Achievements

The release kinetics of fluorescent substances encapsulated in spherical vesicles of lipid bilayers were measured in the presence of pesticides, and release kinetic parameters (release rate constant, initial rate of release) were calculated. Using the release kinetic parameters obtained from the bilayers with different degrees of unsaturation of the phospholipid acyl chains, we were able to obtain multiple regression equations to predict the toxicity of the six pesticides. Furthermore, thermal analysis of the interactions between the lipid bilayers and the pesticides was performed to infer the nature of the interactions. We also measured the basic properties of bilayers with branched acyl chains, and obtained guidelines for the design of new model cell membranes. On the other hand, for the immobilization of lipid bilayers, we investigated their embedding in gels on electrodes, but were unable to select an effective gel material.

Translated with www.DeepL.com/Translator (free version)

Free Research Field

化学工学

Academic Significance and Societal Importance of the Research Achievements

限定的ではあるが，脂質二分子膜-農薬間の相互作用に基づく農薬毒性の予測式が得られたことにより，生物や細胞を用いない簡便な毒性評価法を提示できた。また，熱分析によって溶解度の小さい農薬と分子集合体である脂質二分子膜との結合に関する熱力学パラメータを測定できたことは，農薬の二分子膜への分配位置や相互作用の解明に成果と考えられる。