Development of prevascularized scaffold using microfabrication technique

2023 Fiscal Year Final Research Report

• Project/Area Number: 21K12625
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 90110:Biomedical engineering-related
• Research Institution: Kyoto University
• Principal Investigator: Kawai Toshiyuki 京都大学, 医学研究科, 助教 (80806828)
• Project Period (FY): 2021-04-01 – 2024-03-31
• Keywords: Bone formation / Angiogenesis / polycaprolactone

Outline of Final Research Achievements

A 3 cm bone defect was created in the femur of a beagle dog, a porous body made of polycaprolactone was inserted, and the femur was fixed with a polyetheretherketone plate. At 8 weeks, the porous group had more blood vessels than the empty group, and the BMP2 group had even more blood vessels than the porous group. The BMP2 group had even more blood vessels than the porous group. Bone formation at 8 weeks tended to be greater in the porosity and BMP2 groups than in the empty group, but the difference was not significant.

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Free Research Field

整形外科学

Academic Significance and Societal Importance of the Research Achievements

critical-sizeの巨大骨欠損部に吸収性の多孔体をscaffoldとして留置することで血管新生を促進することが示された。そこにさらにBone Morphogenetic Protein 2を添付することで血流がさらに増加するというデータが得られ、現在臨床で問題となっている巨大な骨欠損の再建の際の深部の血流・栄養不足の問題に対処する際の有効な手立てへと繋がる可能性がある。今回は骨形成量が充分得られなかったが、周囲からの血流を誘導するための人工血管構造を作成し、動脈と吻合することで深部への更なる血流改善、骨形成誘導が得られる可能性がある。