Effects of mechanical stress on growth and differentiation of DFAT cell

2023 Fiscal Year Final Research Report

• Project/Area Number: 21K12632
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 90110:Biomedical engineering-related
• Research Institution: Nihon University
• Principal Investigator: KATAOKA Noriyuki 日本大学, 工学部, 教授 (20250681)
• Co-Investigator(Kenkyū-buntansha): 松本 太郎 日本大学, 医学部, 教授 (50366580)
• Project Period (FY): 2021-04-01 – 2024-03-31
• Keywords: DFAT細胞 / 再生医療 / メカニカルストレス

Outline of Final Research Achievements

DFAT cells are induced into cells with properties similar to mesenchymal stem cells by culturing mature adipocytes in a ceiling culture method. In this study, we fabricated an apparatus to apply mechanical stimuli to DFAT cells and developed a system to apply shaking and stretching stimuli simultaneously. The results of this study show that we succeeded in developing a system for simultaneous loading of extensional and flow stimuli to cells, and that cell proliferation was slightly suppressed up to 48 hours after applying only extensional stimuli to DFAT cells. On the other hand, when cells were subjected to simultaneous loading of extension and flow stimuli, cells elongated and cellular senescence was suppressed. Further investigation of loading time and loading pattern will be conducted in the future.

Free Research Field

メカノバイオロジー

Academic Significance and Societal Importance of the Research Achievements

DFAT細胞はiPS細胞やES細胞などの他の多能性細胞と比較して安価、安全、かつ取得が容易な点などから、再生医療への活用が期待されている。現在、DFAT細胞は骨、軟骨、脂肪、平滑筋、血管への分化が確認されているが、機械的刺激負荷によってその増殖や分化を制御出来る可能性があり、再生医療に大きな寄与が可能であると考えられる。本研究の結果は、DFAT細胞の再生医療への応用のみならず、他の幹細胞を用いた再生医療、細胞培養技術全般にも適用可能で大きな学術的・社会的意義がある。