2022 Fiscal Year Research-status Report
Cellular resolution macro analysis tools for functional and structural brain imaging
| Project/Area Number |
21K12665
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| Research Institution | Hiroshima University |
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Principal Investigator |
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| Project Period (FY) |
2021-04-01 – 2024-03-31
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| Keywords | optical imaging / 3d brain imaging / fluorescence microscopy / neuroinformatics |
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| Outline of Annual Research Achievements |
The purpose of the proposed research was to develop open source microscopy tools for 3D brain imaging using novel light source for fluorescence microscopy in the deep ultraviolet spectrum.
In FY2021, a prototypical fluorescence microscope for automated serial slicing and high resolution widefield block-face imaging across the whole brain or tissue block of interest was successfully developed. A manuscript submitted on the technical aspects is currently in second phase of revision.
In FY2022, the applications for quantitative analysis was carried out on normal mouse brains. The study is running smoothly as expected.
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| Current Status of Research Progress |
Current Status of Research Progress
2: Research has progressed on the whole more than it was originally planned.
Reason
In FY2022, tools for quantitative analysis of the cytoarchitecture of the mouse brains was developed using the microscope developed. A technical paper is under final phase of revision to be considered for publication. Further applications of functional imaging are under consideration.
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| Strategy for Future Research Activity |
The plans for the phase3 (FY2023) of the 3-dimensional (3D) fluorescence microscopy for brain imaging are as follows:
In FY2023, the chief focus would be consolidate all 3D microscopy based tools developed using deep ultraviolet microscope to be put into the open domain as the ‘Do-It-Yourself’ 3D microscope for micro-macro analysis so as to enable smaller neuroscience labs with minimal resources to set-up their own version of 3D microscope for a community based approach towards elucidating brain connectivity.
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| Causes of Carryover |
In the last financial year (FY2022), budget reserved for conferences were not utilized as expected to due to the still ongoing COVID pandemic situation. Moreover, these funds were reserved towards covering the publication costs which is expected in FY2023.
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