2023 Fiscal Year Final Research Report
Promote IVF embryo development by reproducing deep body temperature fluctuations in pregnant mice.
| Project/Area Number |
21K12672
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 90110:Biomedical engineering-related
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| Research Institution | Nihon University |
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Principal Investigator |
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| Project Period (FY) |
2021-04-01 – 2024-03-31
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| Keywords | 受精卵 / 培養温度 / 体温 / ゆらぎ / 月経 / 基礎体温 |
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| Outline of Final Research Achievements |
In pregnant female mice, a 0.6℃ increase in body temperature during the light phase (resting) significantly decreased the circadian rhythm. The random fluctuation component increased in complexity while variability decreased. In adult women, fluctuating changes in abdominal skin temperature at bedtime can be used to determine the follicular, ovulatory, and luteal phases of the cycle. Furthermore, the highest abdominal skin temperature reading can be used as an indicator to draw a basal body temperature chart equivalent to the conventional sublingual temperature measurement. Finally, when the embryo was subjected to a culture condition with 24-hour fluctuation cycle, the number of embryos classified as GOOD and FAIR in morphological quality increased 1.8-fold, while the number of embryos classified as POOR decreased.
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| Free Research Field |
生体医工学
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| Academic Significance and Societal Importance of the Research Achievements |
ヒト生殖補助医療では，卵子は受精後の数日間を37℃一定の人工的な体外培養環境で発育した後に母胎に移植される．一方で，受精卵本来の培養環境である母親の深部体温はゆらいでおり．この体温変動を模倣することでより卵子に優しい培養環境を提供できる可能性がある．しかし，これまでに女性の体内深部温度のゆらぎが詳細に調べられたことは無かった．妊娠した雌マウスにのみ確認できた体温変動は，胚盤胞がまだ子宮に着床していない受精後５日以内に妊娠を判別できる可能性を示す．さらに，ゆらぎのある体外培養温度は受精卵品質を向上させる可能性が示されたことから，体外受精におけるさらなる着床率向上に貢献することが期待される．
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