Creation of in vitro 3D glioblastoma tissue using porous highly functional hydrogel

2023 Fiscal Year Final Research Report

• Project/Area Number: 21K12679
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 90120:Biomaterials-related
• Research Institution: Hokkaido University
• Principal Investigator: WANG LEI 北海道大学, 化学反応創成研究拠点, 特任助教 (70637975)
• Co-Investigator(Kenkyū-buntansha): 谷川 聖 北海道大学, 化学反応創成研究拠点, 特任助教 (00823353)
• Project Period (FY): 2021-04-01 – 2024-03-31
• Keywords: 癌 / 癌幹細胞 / 脳腫瘍 / ハイドロゲル / シグナル

Outline of Final Research Achievements

In this study, we use a highly functional porous hydrogel to create a GSC niche and reconstruct GBM tissue that mimics human brain lesions. We will also elucidate the mechanism by which the GSC niche controls and maintains GSCs, and aim to develop GSC-specific treatments based on this. Because treatment resistance and recurrence are caused by the repopulation of GBM cancer stem cells (GSCs), it is necessary to understand the nature of GSCs and develop treatments to eradicate GSCs. Applicants have developed a rapid, efficient, and highly reproducible stem cell induction method using Hokkaido University's proprietary high-performance polymer soft wet gel. In this study, we develop this guidance method, create a GSC niche using a highly functional porous hydrogel, and reconstruct GBM tissue that mimics human brain lesions. We will also elucidate the mechanism by which the GSC niche controls and maintains GSCs, and aim to develop GSC-specific treatments based on this.

Free Research Field

病理学

Academic Significance and Societal Importance of the Research Achievements

本研究は高機能高分子ソフト&ウェットゲルを用いて高速・効率的かつ再現性が高い幹細胞の誘導法を開発した。さらにPorous化した高機能ハイドロゲルを用いて、GSCニッシェを創出することでヒトの脳内病変を模倣したGBM組織を再構築し、GBM幹細胞の性質の解明に貢献した。よって、本研究は次世代の個別化医療の実現に期待される。