2023 Fiscal Year Final Research Report
Development of therapeutic application of beating cardiomyocytes derived from human adipose tissue for treating heart failure
| Project/Area Number |
21K12681
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 90120:Biomaterials-related
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| Research Institution | Kanazawa University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
薄井 荘一郎 金沢大学, 附属病院, 講師 (50507043)
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| Project Period (FY) |
2021-04-01 – 2024-03-31
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| Keywords | 皮下脂肪間質細胞 / 心筋分化 / 再生治療 |
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| Outline of Final Research Achievements |
Subcutaneous fat tissue was collected from patients undergoing elective surgery. Fat tissue was digested using collagenase and centrifuged to isolate human SVF. After seeding SVF on a culture dish, the "myocardial induction gene" MEF2C was transduced using a lentiviral vector on day 7 and analyzed on day 28. Transduction of MEF2c alone into human SVF did not induce differentiation into beating cardiomyocytes. The different findings in transdifferentiation of SVF between mice and humans were attributed to 1) reduced quality of the subcutaneous fat of the donor due to aging and metabolic diseases and 2) interspecies differences in key genes. Plans are ongoing to clarify the cardiac function-improving effect of "myocardial differentiation-inducing gene transfer" SVF administration in a rat model of chronic ischemic heart failure.
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| Free Research Field |
循環器内科学
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| Academic Significance and Societal Importance of the Research Achievements |
申請者は、マウスのSVFを自律拍動心筋細胞(Beating SVF)に再現性をもって分化させる実験系の確立に成功し、Mef2cが心筋分化を制御することを明らかにした。本研究は、安全で汎用性が高い生体材料（SVF）に、遺伝子導入を行うことにより新しい治療的付加価値が与えられることを示すことで、慢性重症心不全をターゲットとした新規心筋再生治療開発へ繋がる研究と位置づけられる。
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