2021 Fiscal Year Research-status Report
A common and potentially prebiotic activating agent for phosphorylation of nucleosides and oligomerization of nucleotides and amino acids
| Project/Area Number |
21K14029
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| Research Institution | Tokyo Institute of Technology |
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Principal Investigator |
蟻 瑞欽 東京工業大学, 地球生命研究所, 研究員 (00860090)
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| Project Period (FY) |
2021-04-01 – 2023-03-31
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| Keywords | amino acids / activating chemistry / peptide synthesis |
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| Outline of Annual Research Achievements |
The proposed cyanogen chloride induced pathway for the synthesis of RNA and peptides includes: 1. phosphorylation of nucleosides to nucleotides; 2. activation of nucleotides to their 5′-phosphorimidazoles for RNA polymerization;3. activation of amino acids to peptides and deprotection. This year, the feasibility of cyanogen chloride activated polymerization of amino acids at step 3 has been demonstrated. It was observed that cyanogen chloride could react with amino acids to afford N-(aminocarbonyl)peptides, and deprotection of the urea group from N-(aminocarbonyl)peptides by NO2 could produce free peptides. These results indicates that the polymerization of RNA (my previous result) and amino acids could occur using cyanogen chloride as activating agent under a single-pot environment.
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| Current Status of Research Progress |
Current Status of Research Progress
2: Research has progressed on the whole more than it was originally planned.
Reason
This project was divided into two stages. Stage 1 is to explore cyanogen chloride mediated phosphorylation of nucleosides to nucleotides, and stage 2 is to assess cyanogen chloride (ClCN) activated polymerization of amino acids to peptides. This project was scheduled to be finished in two years. In the original plan, stage 1 would be finished last year. Since I already had some primary result of stage 2, so I changed the sequence of the project plan and continued to explore the feasibility of stage 2 and almost finished the goals of stage 2.
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| Strategy for Future Research Activity |
Most of the experiments in the stage 2 have been finished last year. I will summary the result and plan to publish the results this year. I will continue to explore the possibility of stage 1.
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| Causes of Carryover |
Due to the influence of Corvid-19, travel and other expenses related to conferences that I was planning to attend have been cancelled.
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