2022 Fiscal Year Research-status Report
A common and potentially prebiotic activating agent for phosphorylation of nucleosides and oligomerization of nucleotides and amino acids
| Project/Area Number |
21K14029
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| Research Institution | Tokyo Institute of Technology |
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Principal Investigator |
蟻 瑞欽 東京工業大学, 地球生命研究所, 研究員 (00860090)
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| Project Period (FY) |
2021-04-01 – 2024-03-31
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| Keywords | amino acids / activating chemistry / peptide synthesis / RNA polymerization / solid-phase synthesis |
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| Outline of Annual Research Achievements |
The proposed cyanogen chloride induced pathway for the synthesis of RNA and peptides includes: 1. phosphorylation of nucleosides to nucleotides; 2. activation of nucleotides to their 5′-phosphorimidazoles for RNA polymerization;3. activation of amino acids to peptides and deprotection. This year, the feasibility of cyanogen chloride activated polymerization of amino acids in the solid-phase peptide synthesis has been demonstrated. We also observed that activation of nucleotides to their 5′-phosphorimidazoles for RNA polymerization and amino acids polymerization can occur in a one-pot synthesis using cyanogen chloride as a common activating agent.
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| Current Status of Research Progress |
Current Status of Research Progress
2: Research has progressed on the whole more than it was originally planned.
Reason
This project was divided into two stages. Stage 1 is to explore cyanogen chloride activated phosphorylation of nucleosides to nucleotides, and stage 2 is to assess cyanogen chloride (ClCN) activated polymerization of amino acids to peptides. This project was scheduled to be finished in two years. In the original plan, stage 1 would be finished last year. Since I already had some primary result of stage 2, so I changed the sequence of the project plan and continued to explore the feasibility of stage 2 and finished the goals of stage 2.
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| Strategy for Future Research Activity |
All the experiments in the stage 2 have been finished. I am writing the paper now to summary the whole results in the stage 2. I will continue to explore the possibility of stage 1 this year.
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| Causes of Carryover |
My kid was born last year, and I need to take care of her, so I don't have much time to do the lab work and travel to the meeting. Next year, I will use the remaining budget to buy the chemicals to continue the experiments in the proposed experiments in stage 1 and pay the open access fee for the submitted paper which summaries the whole results in the stage 2.
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