2021 Fiscal Year Research-status Report
Computational design of recognition peptide based plasmonic biosensor for small molecules detection
| Project/Area Number |
21K14169
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| Research Institution | Okayama University |
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Principal Investigator |
WANG JIN 岡山大学, ヘルスシステム統合科学学域, 助教 (10870975)
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| Project Period (FY) |
2021-04-01 – 2024-03-31
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| Keywords | peptide / odorant binding protein / docking / organic receptors |
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| Outline of Annual Research Achievements |
This research focuses on developing peptide recognition elements for small volatile organic molecules detection, which are diseased-related compounds. Benzaldehyde and acetone binding peptides have been derived from the odorant binding protein. Through molecular docking and simulation, the binding pocket and residues are determined. The amino acids SER and GLN played an important role in interaction with
benzaldehyde by forming hydrogen bond and THR with acetone.
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| Current Status of Research Progress |
Current Status of Research Progress
2: Research has progressed on the whole more than it was originally planned.
Reason
The amino acids sequence for small volatile organic compounds benzaldehyde and acetone has been obtaied through the powerful computational visualization from the most sensitive mosquito odorant binding protein (mOBP). The approach addressed promising strategy for further development of recognition elements for odor sensing. Initial investigation on computation of organic receptors for small molecules has been published in the journal of the electrochemistry society. Based on the original research plan, the results on the peptide will be summarized and presented on the conference.
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| Strategy for Future Research Activity |
After obtain the binding peptide, the peptide modified plasmonic sensor and quartz crystal
microbalance sensor will be performed for further evaluation of the binding affinity of designed peptide receptors. Meanwhile, the selectiviy of the peptides will be also investigated.
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| Causes of Carryover |
fabricate the sensor chip and consumption of related reagents, and meeting and paper fee.
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