2022 Fiscal Year Research-status Report
Complex research on the allelopathic effect of macrophytes on cyanobacteria and the effect of microplastics on macrophytes, cyanobacteria, and macrophytes allelopathy on cyanobacteria
| Project/Area Number |
21K14248
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| Research Institution | Saitama University |
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Principal Investigator |
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| Project Period (FY) |
2021-04-01 – 2024-03-31
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| Keywords | Adsorption / Antioxidants / Macrophytes / Microplastics / Morphology / Naoplastics / Physiology / Interactions |
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| Outline of Annual Research Achievements |
The effect of microplastics (MPs) on the M. roraima response to M. aeruginosa exposure was studied. The effect of MPs on cyanobacteria was also tested. The adsorption of microplastics to the M. roraima roots was unchanged in M. aeruginosa co-culture compared to the monoculture. The cyanobacteria affected the growth and physiology of M. roraima than monoculture. Under the co-existence condition, M. aeruginosa growth was reduced at lower MP concentration and increased at higher concentrations. The oxidative stress increased at higher MP concentrations. Nanoplastic exposure experiments revealed that nano-plastics also have impacts on M. roraima and M. aeruginosa growth and physiology. Fieldwork found that almost all the freshwater resources we tested were contaminated with microplastics.
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| Current Status of Research Progress |
Current Status of Research Progress
1: Research has progressed more than it was originally planned.
Reason
We could manage the budget to extend the experiment for the nano-plastics effect on macrophytes and microplastics. Most of the required resources for extending the research were available in the laboratory. We completed experiments with nano-plastics and filed investigations for microplastic contaminations. Currently, two manuscripts are being prepared for Microplastic's impacts on cyanobacterial allelopathy on macrophytes and the impact of microplastics on cyanobacteria.
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| Strategy for Future Research Activity |
Current findings proved that microplastics and nano plastics both have significant effects on the macrophytes, cyanobacteria, and their interactions. We want to further extend the research by applying machine learning techniques to count the number of particles attached to the tissues and to easily distinguish cyanobacteria stress response to plastic exposure. The long-duration exposure-response study is continuing as the experiment had to be repeated several times due to contaminations. Two manuscripts are under preparation and expected to publish this year.
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| Causes of Carryover |
We decided to keep the amount of ￥242,869 for the next fiscal year as we could not finish the manuscripts within the fiscal due to the repetition of two experiments due to sample contaminations; therefore the amount will be used as English correction charges and manuscript publication charges.
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