2022 Fiscal Year Final Research Report
Evaluation of the transmission and conservation of antibiotic resistance on ESBL-producing genes in water environment
Project/Area Number |
21K14274
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Research Category |
Grant-in-Aid for Early-Career Scientists
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Allocation Type | Multi-year Fund |
Review Section |
Basic Section 22060:Environmental systems for civil engineering-related
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Research Institution | Yamagata University |
Principal Investigator |
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Project Period (FY) |
2021-04-01 – 2023-03-31
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Keywords | 腸内細菌科細菌 / ESBL / 都市下水 / プラスミド / 薬剤耐性 |
Outline of Final Research Achievements |
The Infections caused by Antimicrobial Resistance Bacteria (ARB) are widely recognized as a major public health problem in the 21st century. Extended-spectrum β-lactamase (ESBL)-producing Enterobacteria, which are resistant to a wide range of antibiotics in beta-lactam group, have been recognized worldwide as a threat causing nosocomial infections. To characterize the ESBL-Ent carried by healthy people, we isolated the bacteria from municipal wastewater and detected genes (bla) cording β-lactamase production. Also, We conducted an in-vitro experiment to evaluate persistence of antimicrobial-resistant bacteria (ARB) and conservation of relevant genes (ARGs) among them, focusing on coliform bacteria producing ESBL isolated in water environment.
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Free Research Field |
土木環境システム
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Academic Significance and Societal Importance of the Research Achievements |
本研究で行ったESBL産生菌の特徴づけにとって、都市下水のモニタリングでは、医療機関で検出されないようなESBL産生菌が、市中で蔓延していることが分かった。同様のモニタリングは国内外を問わず広く適用可能であり,ESBL産生菌以外の耐性菌についても、市中での蔓延状況を明らかにすることができると考えている。また、in vitro試験を用いたESBL産生菌の生残性と保存性の評価結果から、腸内細菌科細菌の中でも環境中での生残性が異なり、それらは耐性遺伝子に依存しないことを明らかとした。この結果は、環境中でのESBL産生菌の挙動や拡散の可能性を議論する上で重要なデータとなり得る。
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