2022 Fiscal Year Final Research Report
Trace detection based on concentrated reaction field in porous particle
Project/Area Number |
21K14650
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Research Category |
Grant-in-Aid for Early-Career Scientists
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Allocation Type | Multi-year Fund |
Review Section |
Basic Section 34020:Analytical chemistry-related
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Research Institution | University of Tsukuba |
Principal Investigator |
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Project Period (FY) |
2021-04-01 – 2023-03-31
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Keywords | 微量計測 / 顕微分光 / マイクロ粒子 / DNA / 細孔性粒子 / タンパク質 |
Outline of Final Research Achievements |
We have demonstrated that horseradish peroxidase (HRP) is concentrated several hundred-fold in silica particles, and single-stranded and duplex DNA are concentrated by approximately a thousand-fold. Using this concentration reaction field, we have shown the ability to quantify hydrogen peroxide at a concentration of 980 nM in a single-particle system. Additionally, we have elucidated the diffusion behavior of single-stranded and duplex DNA within the pores of silica particles. Single-stranded DNA tends to be distributed as aggregates within the particles, while duplex DNA readily dissociates inside the particles. By concentrating hairpin-type DNA within the particles and detecting target DNA based on the cleavage reaction of hairpin NDA, we have demonstrated the quantification of target DNA at a concentration of 10 nM in a single-particle system.
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Free Research Field |
分析化学
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Academic Significance and Societal Importance of the Research Achievements |
本研究では、シリカ粒子内に濃縮された分子を利用した計測原理を確立させた。その濃縮倍率などを定量的に議論している研究例はなく、学術的に意義深い。また、単一粒子系で顕微分光法によって計測を行うことにより、高感度な計測が可能となる。本計測法はレーザートラップやマイクロデバイスと親和性が高く、組み合わせることで簡便な検出システムを構築できるため、社会的貢献も見込める。また、DNAの粒子内の拡散挙動はクロマトグラフィーの分離機構を明らかにするための基礎知見となり、当該分野での学術的寄与を期待できる。
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