2022 Fiscal Year Annual Research Report
Revealing oxidative stress in neurodegenerative brain by hydroxyl-radical-focused imaging and proteomics
| Project/Area Number |
21K14752
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| Research Institution | Kyoto University |
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Principal Investigator |
朱 浩 京都大学, 工学研究科, 助教 (90874545)
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| Project Period (FY) |
2021-04-01 – 2023-03-31
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| Keywords | reactive oxygen species / hydroxyl radical / conditional proteomics |
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| Outline of Annual Research Achievements |
This research develops the first example of hydroxyl-radical-responsive protein labeling reagents for fluorescence imaging and proteomics, which aims at the comprehensive understanding of hydroxyl radical in living systems by unraveling its localization and surrounding/associated proteins. In the first year, a series of phenol/aniline-derived chemical probes have been designed and synthesized and in vitro protein labeling experiments successfully identified hydroxyl-radical-selective probes. In the second year, we moved to live-cell assays with synthesized cell-permeable probes. The probe structure was slightly modified to reduce the background labeling in living cells. Preliminary results have demonstrated its availability in ROS conditional protein labeling in activated macrophages.
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