2023 Fiscal Year Final Research Report
Comprehensive studies for the biotechnological production of phenylethanoid glycosides.
| Project/Area Number |
21K14788
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| Research Category |
Grant-in-Aid for Early-Career Scientists
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| Allocation Type | Multi-year Fund |
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| Review Section |
Basic Section 38030:Applied biochemistry-related
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| Research Institution | Institute of Physical and Chemical Research |
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Principal Investigator |
Fuji Yushiro 国立研究開発法人理化学研究所, 環境資源科学研究センター, 特別研究員 (90847815)
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| Project Period (FY) |
2021-04-01 – 2024-03-31
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| Keywords | フェニルエタノイド配糖体 / 生合成 / アクテオシド / ゴマ |
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| Outline of Final Research Achievements |
Phenylethanoid glycosides (PhGs) are compounds based on C6-C2 glucose glycoside. Acteoside, which is representative PhG, is present in many medicinal plants and is expected to be used as a medicine because of its various pharmacological effects. However, mass production systems have not yet been established. Thus, a study was conducted to elucidate the mechanism of acteoside biosynthesis.
As a result, we selected candidate genes for the acteoside biosynthetic pathway by transcriptome analysis using a sesame cell culture system and an elicitation for acteoside-induced production system. Furthermore, we identified glycosyltransferases and acyltransferases important for the structural formation of PhGs by functional analysis using recombinant proteins.
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| Free Research Field |
植物生化学
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| Academic Significance and Societal Importance of the Research Achievements |
フェニルエタノイド配糖体は様々な薬理作用を示し，医薬品としての利用が期待されるものの量産化が課題であった．本研究の遂行により，フェニルエタノイド配糖体の骨格形成に重要な配糖体化酵素やアシル化転移酵素が明らかとなり，バイオ生産に向けた取り組みに大きく貢献できるものと考えられる．
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