Genomic comparisons of cryptic species to investigate interspecific hybridization hotspots in oval squid

2023 Fiscal Year Final Research Report

• Project/Area Number: 21K14897
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 40030:Aquatic bioproduction science-related
• Research Institution: Regional Fish Institute, Ltd (R&D Division) (2022-2023); The University of Tokyo (2021)
• Principal Investigator: TOMANO Satoshi リージョナルフィッシュ株式会社(研究開発部), 研究開発部, 研究員 (30866570)
• Project Period (FY): 2021-04-01 – 2024-03-31
• Keywords: 頭足類 / 種間交雑 / アオリイカ / 遺伝的多様性 / 生殖隔離

Outline of Final Research Achievements

The purpose of this study was to examine the status of reproductive isolation and introgression among the three species of oval squid through interspecific hybridization tests using artificial insemination and breeding experiments. First of all, it took time to start up the experiment until the second year of the project due to the spread of the coronavirus epidemic and the change of the representative's affiliation. However, we established an artificial insemination method for the oval squid and were able to achieve a fertilization success rate of about 60%. This result is high compared to previous studies and represents a significant advance in artificial insemination technology for squids. Furthermore, artificial insemination among multiple species of the oval squid genus was investigated, and it was suggested that hybridyzation may occur.

Free Research Field

水圏生命科学

Academic Significance and Societal Importance of the Research Achievements

アオリイカにおける人工授精方法を確立し、6割程度の受精成功度を得ることができた。この結果は既往研究に比較して非常に高い値であり，アオリイカ類の人工授精技術を大きく発展させたといえる。さらにアオリイカ属複数種間での人工授精を試み、授精がおきることが示唆された。これは近年注目されているアオリイカ類の完全養殖や、養殖に適した系統の作出に非常に有益である。さらに、本研究で得られた知見の一部は、4件の国際誌、国際学会および国内学会に発表する成果となった。これはイカ類の繁殖生態や集団遺伝学的研究に大きく寄与するものといえる。