Identification of clostridia associated with butyric acid fermentation in silage

2023 Fiscal Year Final Research Report

• Project/Area Number: 21K14967
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 42010:Animal production science-related
• Research Institution: National Agriculture and Food Research Organization
• Principal Investigator: Kobayashi Hisami 国立研究開発法人農業・食品産業技術総合研究機構, 畜産研究部門, 主任研究員 (80609701)
• Project Period (FY): 2021-04-01 – 2024-03-31
• Keywords: サイレージ / 酪酸発酵 / 芽胞形成細菌

Outline of Final Research Achievements

The objectives of this study were 1) to clarify the diversity of Clostridium spp. in silage, fermented forage for livestock and 2) to identify Clostridium spp. associated with the low quality of silages due to butyric acid fermentation. As the results of research for 1), the novel Clostridium species, which the name Clostridium zeae and Clostridium folliculivorans was proposed. In addition, the taxonomic status of the species Clostridium methoxybenzovorans was clarity. On the result of 2), grass silage inoculated with Clostridium spp. isolated silage contained high butyric acids. It suggested that these Clostridium spp. have potential to be associated with butyric acid fermentation in grass silage.

Free Research Field

飼料調製

Academic Significance and Societal Importance of the Research Achievements

本研究課題により得られた新たなClostridium属細菌種およびClostridium属細菌種の再分類についての成果は、畜産学におけるサイレージの低質化メカニズムの解明への貢献に加えて、微生物学における系統分類研究としての学術的意義としての価値がある。また、実験室規模におけるClostridium属細菌接種試験により選定されたサイレージ酪酸発酵に関与する細菌種を実規模サイレージでの探索にステップアップするとともに、Clostridium属細菌の制御方法を検討していくことで、良質なサイレージ調製技術の確立による畜産業の発展が見込まれる。