Devleopment of new xenogeneic hematopoietic stem cell chimeras by in utero hematopoietic stem cell transplantation

2022 Fiscal Year Final Research Report

• Project/Area Number: 21K15003
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 42040:Laboratory animal science-related
• Research Institution: University of Tsukuba
• Principal Investigator: Jeon Hyojung 筑波大学, 医学医療系, 助教 (20837091)
• Project Period (FY): 2021-04-01 – 2023-03-31
• Keywords: 造血幹細胞 / 経胎盤移植

Outline of Final Research Achievements

This study aimed to create xenogeneic hematopoietic stem cell chimeras using xenogeneic hematopoietic stem cells. We established a method that had little effect on the survival rate of the fetus after transplantation using intra-placental transplantation. We used hematopoietic deficient mice Runx1 cKO::Tie-2-cre as recipients. We found that the transplanted cells engrafted successfully in the fetuses without needing pre-transplant conditioning and that the transplanted fetuses survived to term and were healthy.

Free Research Field

発生学

Academic Significance and Societal Importance of the Research Achievements

本研究の成果は、従来の造血幹細胞移植研究で問題となっている骨髄破壊的前処置を必要とせず、ドナー細胞の生着率を高めることができる新しい造血幹細胞移植法として学術的に重要である。さらに、新たな実験動物モデルの作成につながる可能性があるため社会的意義が大きいと考えられる。