Investigation of translational factor pool formed around the ribosomal P-stalk

2023 Fiscal Year Final Research Report

• Project/Area Number: 21K15015
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 43010:Molecular biology-related
• Research Institution: University of the Ryukyus
• Principal Investigator: Imai Hirotatsu 琉球大学, 医学(系)研究科(研究院), 助教 (00866668)
• Project Period (FY): 2021-04-01 – 2024-03-31
• Keywords: 翻訳 / リボソーム / RNA

Outline of Final Research Achievements

In this project, we focused on the ribosomal P-stalk, which consists of ribosomal proteins P0, P1, and P2, that form translational GTPase factor pool for efficient translation. We tried to identify novel P-stalk binding proteins and to elucidate the general role of the P-stalk in translation. Using proximity labeling, we found that not only translational GTPase factors such as EF1A and EF2, but also translation initiation factors, aminoacyl-tRNA synthetase, RNA-binding proteins, ribosome-hibernation proteins and functionally unknown proteins localize near P-stalks. This finding is important as basic information for understanding of the multiple roles of P-stalks in translation.

Free Research Field

分子生物学

Academic Significance and Societal Importance of the Research Achievements

遺伝情報の翻訳は最も基本的な細胞内プロセスの一つであり、その反応には多種多様な分子が関わる。本研究では、翻訳に必須である分子装置Pストークのはたらきを理解するための情報的基盤を構築した。本研究を足がかりとした「翻訳が滞りなく進行するための分子基盤」の解明により、（1）細胞システムの成り立ちの理解と、（2）翻訳の破綻が原因で生じる疾患の原因究明や治療法の確立において重要な知見が得られるようになると期待される。