Next generation TnDR for proteome-wide profiling of co-translatinoal protein dynamics

2022 Fiscal Year Final Research Report

• Project/Area Number: 21K15020
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 43010:Molecular biology-related
• Research Institution: Kyoto Sangyo University
• Principal Investigator: Fujiwara Keigo 京都産業大学, 生命科学部, 研究員 (10814907)
• Project Period (FY): 2021-04-01 – 2023-03-31
• Keywords: 新生鎖 / 翻訳アレスト / MifM / Tn-seq / co-translational / 枯草菌

Outline of Final Research Achievements

This study developed a fundamental framework for TnDR-seq that enables the comprehensive detection of co-translational protein dynamics at the proteome level. This was achieved by utilizing a force-sensing arrest peptide with a transposon and deep-sequencing system. Our findings indicate a shared pattern of co-translational movement among secretory proteins and membrane proteins. Additionally, distinct co-translational dynamics have been observed for several cytosolic soluble proteins. Moreover, through bioinformatic analysis, novel candidate arrest peptides have been identified.

Free Research Field

分子生物学

Academic Significance and Societal Importance of the Research Achievements

本研究のTnDR-seqを活用することで、今後、co-translationalな成熟・局在化の普遍的な法則性や、それに対する環境変動の影響などを見出せるようになると期待できる。それらはタンパク質科学における基礎的な重要知見となり、タンパク質生合成における様々な細胞内現象の理解を補助したり、有用タンパク質の効率的な大量生産に貢献したりすることで、種々の社会的問題の解決策へつながることが期待される。